Table 1 Effect of different hydrolysis conditions (temperature and time) on recovery of the tested keyhole limpet hemocyanin (KLH) protein sample
From: Determination of immunogenic proteins in biopharmaceuticals by UHPLC–MS amino acid analysis
Expected concentration [mg mL−1] | Determined concentration [mg mL−1] | Recovery [%] | |
|---|---|---|---|
110 °C/20 h | 20 | 19.56 | 97.8 |
110 °C/24 h | 20 | 19.64 | 98.2 |
110 °C/28 h | 20 | 19.14 | 95.7 |
120 °C/20 h | 20 | 20.00 | 100.0 |
120 °C/24 h | 20 | 20.12 | 100.6 |
120 °C/28 h | 20 | 19.84 | 99.2 |
130 °C/20 h | 20 | 19.76 | 98.8 |
130 °C/24 h | 20 | 20.30 | 101.5 |
130 °C/28 h | 20 | 19.74 | 98.7 |