Table 1 Intra-and inter-day assay precision and accuracy for the determination of 5-HT in RPMI medium 1640
ASSAY | Nominal concentration (μg/mL) | Mean measured concentration (μg/mL) | CV (%) | Accuracy (%) |
|---|---|---|---|---|
Intra‐assay (n = 5) | 0.1 | 0.093 | 2.45 | 92.96 |
0.3 | 0.305 | 1.12 | 101.71 | |
4 | 4.060 | 1.25 | 101.50 | |
7.5 | 7.493 | 0.32 | 99.90 | |
Inter‐assay (n = 15) | 0.1 | 0.092 | 4.63 | 92.02 |
0.3 | 0.310 | 3.82 | 103.28 | |
4 | 3.981 | 4.69 | 99.53 | |
7.5 | 7.518 | 0.62 | 100.24 |