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Table 2 Back-calculated concentrations of calibration standards

From: A simple, efficient and rapid HPLC–UV method for the detection of 5-HT in RIN-14B cell extract and cell culture medium

Back calculation

(n = 3)

Nominal concentration (μg/mL)

0.1

0.2

0.4

0.8

1.25

2.5

5

10

Mean

0.105

0.209

0.413

0.808

1.272

2.540

5.086

10.137

SD

0.004

0.008

0.010

0.018

0.019

0.039

0.075

0.186

CV (%)

3.44

4.01

2.45

2.20

1.46

1.54

1.48

1.85

Accuracy (%)

104.94

104.70

103.30

100.97

101.77

101.60

101.71

101.37