A simple, efficient and rapid HPLC–UV method for the detection of 5-HT in RIN-14B cell extract and cell culture medium

Table 3 5-HT stability in spiked medium samples (n = 5)

Storage condition	Nominal concentration (μg/mL)	Mean measured concentration (μg/mL) ± SD	95% confidence intervals	Accuracy (%)
Freshly prepared samples	0.3	0.305 ± 0.003	0.301–0.309	101.70
Freshly prepared samples	7.5	7.494 ± 0.024	7.463–7.522	99.92
24 h at room temperature	0.3	0.299 ± 0.004	0.295–0.304	99.80
24 h at room temperature	7.5	7.514 ± 0.022	7.487–7.542	100.19
Three freeze–thaw cycles	0.3	0.297 ± 0.003	0.294–0.300	99.03
Three freeze–thaw cycles	7.5	7.516 ± 0.011	7.502–7.530	100.21
60 days at 4 ℃	0.3	0.310 ± 0.001	0.309–0.311	103.03
60 days at 4 ℃	7.5	7.518 ± 0.004	7.512–7.524	100.24

ISSN: 2661-801X