Parameter | No. of theoretical plates (N) | Capacity factor (k′) | Tailing factor (Tf) | |||
---|---|---|---|---|---|---|
Column (A) | Column (B) | Column (A) | Column (B) | Column (A) | Column (B) | |
Column temperature °C | ||||||
25 °C | 4354.9 | 1014.2 | 1.147 | 4.638 | 0.475 | 0.629 |
40 °C | 2950.3 | 756.4 | 1.057 | 4.404 | 0.506 | 0.772 |
50 °C | 2620.8 | 677.9 | 0.989 | 4.208 | 0.471 | 0.73 |
65 °C | 1895.1 | 468.9 | 0.871 | 3.792 | 0.468 | 0.679 |
pH of mobile phase | ||||||
2.5 | 4354.9 | 1014.2 | 1.147 | 4.638 | 0.475 | 0.629 |
3.5 | 3981.2 | 1089 | 1.145 | 4.667 | 0.526 | 0.588 |
5 | 4617.2 | 1066.9 | 1.145 | 4.667 | 0.455 | 0.572 |
Type of organic modifier of Conc 40% (v/v) | ||||||
Acetonitrile | 4390.4 | 1004.5 | 1.236 | 4.667 | 0.446 | 0.679 |
Methanol | 5073.4 | 1214.5 | 1.179 | 4.738 | 0.474 | 0.664 |
Ethanol | 3342 | 925.4 | 1.175 | 4.708 | 0.481 | 0.65 |
Ratio organic modifier: mobile phase (Acetonitrile) (v/v) | ||||||
40:60 | 4354.9 | 1100.3 | 1.147 | 4.696 | 0.475 | 0.677 |
60:40 | 1925.3 | 1004.5 | 1.173 | 4.667 | 0.453 | 0.679 |
80:20 | 7501.7 | 1648.5 | 1.187 | 4.75 | 0.468 | 0.647 |
90:10 | 8304.6 | 2064.8 | 1.19 | 4.792 | 0.458 | 0.653 |
Effect of flow rate (mL/min) | ||||||
0.3 | 1041 | 8.196 | 0.816 | |||
0.5 | 1090.7 | 4.613 | 0.613 | |||
1 | 5093.6 | 1.53 | 0.287 | |||
1.2 | 1117.9 | 1.175 | 0.465 |