Table 1 UHPLC–Q-TOF-MS data of identified alkaloids from fruits of M. microcarpa
Peak no. | TR (min) | Theoretical mass (m/z) | Molecular formula | Error (ppm) | Observed mass (m/z) | Fragment ions of MS2 (m/z) | Identified compounds |
|---|---|---|---|---|---|---|---|
1 | 2.296 | 372.1805 | C21H25NO5 | 1.21 | 372.1801 | 165 (20), 181 (10), 190 (100), 191 (24), 192 (42), 208 (29), 338 (12), 354 (23), 372 (35) | Demethylatedmuramine |
2 | 4.549 | 354.1336 | C20H19NO5 | 0.56 | 354.1334 | 149 (39), 165 (17), 188 (90), 189 (100), 190 (7), 206 (17), 336 (11) | Protopine |
3 | 5.564 | 386.1962 | C22H27NO5 | − 3.64 | 386.1976 | 222 (91), 386 (100) | Muramine |
4 | 5.676 | 370.1649 | C21H23NO5 | − 3.79 | 370.1663 | 149 (10), 165 (15), 188 (34), 189 (44), 190 (9), 204 (100), 222 (37), 352 (22) | Allcryptopine |
5 | 6.691 | 370.1649 | C21H23NO5 | − 1.09 | 370.1653 | 165 (15), 181 (19), 188 (100), 189 (47), 190 (9), 206 (32), 336 (11), 352 (21) | Cryptopine |
6 | 6.747 | 332.0923 | C20H14NO4 | − 0.35 | 332.0924 | 274 (24), 304 (30), 317 (27), 332 (100) | Sanguinarine |
7 | 6.916 | 348.1236 | C21H18NO4 | 2.54 | 348.1227 | 290 (20), 304 (53), 318 (42), 332 (100), 333 (39), 348 (31) | Chelerythrine |
8 | 15.932 | 717.2807 | C42H40N2O9 | 1.34 | 717.2797 | 348 (100), 349 (5) | (±)-Macleayin G |
9 | 18.749 | 364.1185 | C21H18NO5+ | 1.37 | 364.1180 | 306 (23), 320 (26), 334 (100), 348 (16), 349 (82), 364 (12) | Dihydrochelirubine |
10 | 3.535 | 386.1587 | 165 (52), 204 (15), 222 (65), 252 (20), 266 (16), 267(25), 283 (68), 306 (26), 309 (37), 325 (66), 386 (29) | Unknown | |||
11 | 6.803 | 453.3436 | 435 (100), 114 (58), 209 (65), 226 (20), 227 (10) 228 (24), 322 (86), 340 (13), 453 (86) | Unknown | |||
12 | 15.819 | 717.2437 | 332 (100), 717 (2) | Unknown | |||
13 | 16.157 | 733.2767 | 348 (100), 733 (15) | Unknown | |||
14 | 18.749 | 749.2125 | 386 (100), 749 (2) | Unknown |