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Table 3 Validation: stability data for thymoquinone in different biomatrixes

From: A bioanalytical UHPLC based method used for the quantification of Thymoquinone-loaded-PLGA-nanoparticles in the treatment of epilepsy

Exposure condition

LQC(290.0 ng/mL or ng g−1)

MQC(1000.0 ng/mL or ng g−1)

HQC (2000.0 ng/mL or ng g−1)

Brain homogenate

Lungs homogenate

Plasma

Brain homogenate

Lungs homogenate

Plasma

Brain homogenate

Lungs homogenate

Plasma

Long term stability; recovery (ng) after storage (− 80 °C)

 Previous day

288.9 ± 0.93

288.6 ± 0.50

288.6 ± 0.54

994.6 ± 13.36

992.6 ± 14.6

993.4 ± 17.4

1993.6 ± 26.4

1992.6 ± 27.8

1989.1 ± 27.5

 30th day

281.2 ± 0.91 (97.33%)

287.1 ± 0.45 (99.48%)

284.1 ± 0.91 (98.44%)

982.4 ± 15.6 (98.77%)

988.6 ± 15.4 (99.60%)

984.6 ± 17.1 (99.11%)

1981.4 ± 24.1 (99.39%)

1988.2 ± 26.1 (99.78%)

1970.4 ± 24.6 (99.06%)

Freeze-thaw stress; recovery (ng) after freeze-thaw cycles (− 80 °C to 25 °C)

 Pre-cycle

287.9 ± 0.67

289.3 ± 0.81

289.1 ± 0.64

993.7 ± 15.6

993.4 ± 14.6

991.5 ± 16.4

1990.4 ± 27.0

1991.8 ± 27.3

1990.6 ± 26.4

 First cycle

284.1 ± 0.90 (98.68%)

286.6 ± 0.86 (99.07%)

288.2 ± 0.74 (99.69%)

985.4 ± 13.9 (99.16%)

988.4 ± 2.46 (99.50%)

987.9 ± 17.5 (99.64%)

1981.7 ± 26.9 (99.56%)

1988.4 ± 26.4 (99.83%)

1981.4 ± 27.4 (99.54%)

 Second cycle

282.3 ± 0.92 (98.05%)

283.4 ± 0.94 (97.96%)

286.47 ± 0.66 (99.09%)

979.4 ± 14.6 (98.56)

985.7 ± 14.2 (99.22%)

986.4 ± 16.3 (99.49%)

1975.4 ± 23.8 (99.25%)

1981.5 ± 25.8 (99.48%)

1975.4 ± 26.1 (99.24%)

 Third cycle

280.3 ± 0.93 (97.36%)

280.1 ± 0.93 (96.82%)

285.94 ± 0.91 (98.91%)

975.6 ± 15.4 (98.18%)

982.4 ± 14.5 (98.89%)

979.9 ± 17.1 (98.83%)

1971.1 ± 24.7 (99.03%)

1974.2 ± 26.7 (99.12%)

1970.9 ± 25.4 (99.01%)

Bench top stability; recovery (ng) at room temperature (25 °C)

 0 h

288.4 ± 0.91

287.8 ± 0.91

288.47 ± 0.71

992.8 ± 14.6

991.4 ± 14.7

989.9 ± 16.8

1992.4 ± 25.4

1989.1 ± 28.1

1991.5 ± 26.7

 24 h

286.3 ± 0.78 (99.27%)

282.3 ± 0.81 (98.09%)

286.44 ± 0.84 (99.30%)

986.4 ± 15.0 (99.36%)

984.6 ± 13.5 (99.31%)

981.4 ± 15.8 (99.14%)

1981.5 ± 27.1 (99.45%)

1977.6 ± 27.5 (99.42%)

1979.1 ± 27.4 (99.38%)

Post processing stability; recovery (ng) after storage in auto sampler (4 °C)

 0 h

289.3 ± 0.34

288.6 ± 0.81

289.21 ± 0.64

993.4 ± 14.9

993.4 ± 14.5

988.7 ± 17.6

1991.4 ± 26.3

1988.9 ± 28.3

1988.3 ± 23.4

 4 h

287.5 ± 0.62 (99.38%)

281.94 ± 0.49 (97.69%)

287.3 ± 0.84 (99.34%)

989.6 ± 13.6 (99.62%)

986.6 ± 13.9 (99.32%)

981.2 ± 18.0 (99.24%)

1983.1 ± 25.7 (99.58%)

1971.5 ± 26.4 (99.13%)

1970.1 ± 22.9 (99.08%)

  1. Values (Mean ± SD) are derived from six replicates. Figures in parenthesis represent analyte concentration (%) relative to time zero. Theoretical contents; LQC: 290.0 ng mL−1; MQC: 1000.0 ng mL−1; and HQC: 2000.0 ng mL−1