HPLC-DAD technique for the quantification of a recently approved anti-diabetic triple combination along with two toxic official impurities: Toxicity confirmation aided by molecular docking application

Table 3 Laboratory prepared mixture results of the current HPLC method for the determination of EMP, LIN, and MET along with MEL and CYG as MET potential official impurities

MIX	EMP	EMP Found	% Recovery ^a of EMP	LIN	LIN Found	% Recovery ^a of LIN	MET	MET Found	% Recovery ^a of MET	MEL	CYG
1	5.00	5.01	100.20	5.00	4.92	98.58	5.00	4.94	98.95	2.50	2.50
2^b	5.00	5.01	100.20	1.00	1.01	101.01	200.00	203.17	101.58	10.00	10.00
3	2.50	2.53	101.21	0.50	0.49	98.94	100.00	100.45	100.45	20.00	20.00
4	4.00	3.96	99.07	3.00	2.96	98.76	10.00	9.86	98.61	4.00	4.00
5	7.00	6.90	98.59	6.00	6.03	100.58	20.00	20.01	100.05	5.00	5.00
	Mean ± SD		99.83 ± 1.022	Mean ± SD		99.58 ± 1.131	Mean ± SD		99.93 ± 1.196

ISSN: 2661-801X