Three developed spectrophotometric methods for determination of a mixture of ofloxacin and ornidazole; application of greenness assessment tools

Table 1 Validation parameters of the developed spectrophotometric techniques to determine of Ofloxacin and Ornidazole

Parameters	Ratio Difference		Mean Centering		Continuous Wavelet Transform
Parameters	OFL	ORN	OFL	ORN	OFL	ORN
Range (µg/mL)	(2–15)	(3–30)	(2–15)	(3–30)	(2–15)	(3–30)
Linearity Regression equation	Y = 0.3361 × + 0.112	Y = 0.1751 × + 0.0139	Y = 0.133 × + 0.016	Y = 0.0691 × + 0.0105	Y = 0.1636X + 0.021	Y = 0.1083X + 0.0262
Slope^a	0.3361	0.1751	0.133	0.0691	0.1636	0.1083
Intercept^a	0.112	0.0139	0.016	0.0105	0.021	0.0262
Correlation coefficient^a	0.9999	0.9998	0.9997	0.9999	0.9997	0.9998
Accuracy^b (mean)	99.91 ± 0.55	99.86 ± 0.91	99.74 ± 0.86	100.29 ± 0.63	100.14 ± 1.20	99.73 ± 0.82
Specificity^c	101.10 ± 0.89	99.97 ± 0.69	100.56 ± 0.86	100.77 ± 0.55	100.99 ± 1.07	101.39 ± 0.94
LOQ (µg/mL)^d	0.98	2.66	1.73	2.30	1.26	2.66
LOD (µg/mL)^e	0.32	0.87	0.57	0.75	0.74	0.88
Precision (± RSD %)
a- Intermediate precision^f	100.01 ± 0.72	100.40 ± 1.08	99.17 ± 0.92	100.43 ± 0.75	99.45 ± 1.21	100.29 ± 0.93
b- Repeatability^g	100.65 ± 0.98	99.75 ± 1.05	99.56 ± 0.58	100.07 ± 0.64	99.95 ± 1.11	100.89 ± 0.95

^aMean of 3 determinations
^bThe average of 3 dissimilar concentrations of ORN and OFL
^cRecovery of dissimilar laboratory prepared mixtures containing varying proportions of ORN and OFL
^dLimit of quantitation is calculated
^eLimit of detection is calculated
^fInterday precision (the RSD of 3 dissimilar concentrations (6, 8, 10 µg/mL for OFL) and (10, 15, 20 for ORN µg/mL) 3 replicates each, on 3 following days
^gIntraday precision (the RSD of 3 dissimilar concentrations (6, 8, 10 µg/mL for OFL) and (10, 15, 20 µg/mL) for ORN) 3 replicates each, within the same day

ISSN: 2661-801X