Table 1 Parameters required for system suitability tests of UPLC method
Parameter | PHL | GUA impurity A | GUA | GUA impurity B | GUA impurity E |
|---|---|---|---|---|---|
Retention time (tR) [min] | 0.46 | 0.79 | 1.14 | 1.77 | 2.41 |
Resolution (Rs) | NA | 2.41 | 2.25 | 2.41 | 2.65 |
Tailing factor (T) | 1.37 | 1.54 | 1.42 | 0.87 | 1.13 |
Selectivity factor (α) | NA | 2.46 | 2.32 | 1.86 | 1.65 |
Column efficiency (N) | 1586.15 | 2037.88 | 1657.65 | 1863.71 | 2966.25 |
Height equivalent to theoretical plate (HETP) [mm] | 0.09 | 0.02 | 0.03 | 0.03 | 0.02 |