From: Ultra-high resolution X-ray structure of orthorhombic bovine pancreatic Ribonuclease A at 100K
Coordinate set (PDB ID) | 6etl | 1kf2 | 7rsa | 3rn3 | 7p4r |
---|---|---|---|---|---|
Precipitating agent | 20 mM sodium citrate at pH 5.3 against 10 µl isopropanol 99.9% isopropanol | 2-propanol, pH 5.2, Liquid diffusion, temperature 298 K | 0.3 M sodium hydroxide, pH 5.3, 2-methyl-2-propanol 43% (v/v) | 30–40% ethanol pH 5.2–5.7 stored in 60% aqueous EtOH | Distilled water. Absolute ethanol |
pH | 5.3 | 5.2 | 5.3 | 5.2–5.7 | 6.5 |
Resolution (Ã…) | 0.85 | 1.10 | 1.26 | 1.45 | 0.85 |
Number of solvent molecules located | 232 | 243 | 183 | 139 | 293 |
R factor | 9.8 | 10.3 | 15.0 | 22.3 | 11.2 |