Earth-friendly micellar UPLC technique for determination of four hypoglycemic drugs in different pharmaceutical dosage forms and spiked human plasma

Table 1 Assay validation sheet of the proposed MUPLC method for the determination of PIO, ALO, GLM, and VLD.

Parameter	PIO	ALO	GLM	VLD
Linearity range (µg/ mL)	1–80	0.1–25	0.25-50	0.3–50
Correlation coefficient (r ⁾	0.9999	0.9999	0.9999	0.9999
Slope	5.6087	8.3759	4.3799	11.0076
Intercept	0.2519	0.1278	-0.6364	-0.3432
SD of intercept	0.5700	0.0839	0.0930	0.1814
LOD (µg/ mL)	0.3353	0.0331	0.0701	0.0544
LOQ (µg/mL)	1.0162	0.1002	0.2124	0.1648
Accuracy (R %)	101.24	98.96	99.79	100.19
Precision
Repeatability (RSD%) ^a Intermediate Precision (RSD%) ^b	1.424 1.822	0.965 1.068	0.552 0.997	0.987 1.256

^a The intra-day relative standard deviation of (5, 10, and 15 µg/mL) of PIO, ALO, GLM, and VLD in triplicate using the proposed method
^b The inter-day relative standard deviation of (5, 10, and 15 µg/mL) of PIO, ALO, GLM, and VLD in triplicate using the proposed method

ISSN: 2661-801X