Earth-friendly micellar UPLC technique for determination of four hypoglycemic drugs in different pharmaceutical dosage forms and spiked human plasma

Table 2 Assay bioanalytical validation parameters of PIO, ALO, GLM, and VLD in human plasma

Parameter	PIO	ALO	GLM	VLD
Linearity range (µg/ mL)	5–25	5–25	5–25	5–25
Correlation coefficient (r)	0.9999	0.9998	0.9997	0.9991
Slope	5.5162	8.4906	4.2208	12.0195
Intercept	1.3595	-0.6215	0.3480	-25.3309
LOD (µg/ mL)	0.2972	0.6271	0.9472	1.6301
LOQ (µg/mL)	0.9007	1.9004	2.8702	4.9398
Accuracy (R %)	99.77	98.66	99.37	100.87
Precision
Repeatability (RSD%) ^a Intermediate Precision (RSD%) ^b	0.295 0.528	0.280 0.781	0.131 1.680	0.891 1.231

^a The intra-day relative standard deviation of (5, 15, and 25 µg/mL) of PIO, ALO, GLM, and VLD in triplicate using the proposed method
^b The inter-day relative standard deviation of (5, 15, and 25 µg/mL) of PIO, ALO, GLM, and VLD in triplicate using the proposed method

ISSN: 2661-801X