Table 1 Regression and validation data for the determination of SER.DMP and DMP by the proposed methods

Wavelength (nm)

220 nm

220 nm

Linearity range

2.5— 25 (μg/mL)

5— 25 (μg/spot)

LOD

0.051(μg/mL)

0.184 (μg/spot)

LOQ

0.165 (μg/mL)

0.202 (μg/spot)

Regression Equation

y ^a = 0.0989 C ^b + 0.0331

y ^a = 0.0824 C ^b + 0.0216

 Correlation coefficient (r)

0.9998

0.9997

 Accuracy (% R) ± SD

100.43 ± 1.02

99.68 ± 1.03

Precision (% RSD)

 Repeatability ^c

1.021

1.025

 Intermediate precision ^d

1.015

1.039

Robustness (% RSD)

 Mobile phase contents ratio (± 2%)

0.812

1.386

 Detection wavelengths (± 2 nm)

1.349

1.526

 Flow rate (± 0.1 ml/min.)

1.778

ـــــــ

Wavelength (nm)

220 nm

220 nm

Linearity range

2.5–25 (μg/mL)

5–25 (μg/spot)

LOD

0.098 (μg/mL)

0.115 (μg/spot)

LOQ

0.186 (μg/mL)

0.237 (μg/spot)

Regression Equation

y ^a = 0.0897 C ^b + 0.0522

y ^a = 0.0905 C ^b + 0.0612

 Correlation coefficient (r)

0.9997

0.9996

 Accuracy (% R) ± SD

100.15 ± 1.1

99.50 ± 1.2

Precision (% RSD)

 Repeatability ^c

1.105

1.160

 Intermediate precision ^d

1.251

1.249

Robustness (% RSD)

 Mobile phase contents ratio (± 2%)

0.812

1.128

 Detection wavelengths (± 2 nm)

1.349

1.630

 Flow rate (± 0.1 ml/min.)

1.778

ـــــــ