Table 2 Determination of SER.DMP in laboratory prepared mixtures with its acidic degradation products by the proposed HPLC and TLC procedures
SER.DMP. (HPLC method) | ||||
|---|---|---|---|---|
Intact (µg/mL) | Acidic degradates (µg/mL) | Degradate % | Intact found (µg/mL) | Recovery % of Intacta |
5 | 20 | 80 | 5.04 | 100.86 |
10 | 15 | 60 | 10.13 | 101.25 |
12.5 | 12.5 | 50 | 12.61 | 100.91 |
15 | 10 | 40 | 14.92 | 99.45 |
20 | 5 | 20 | 19.90 | 99.52 |
Mean | 100.40 | |||
RSD% | 0.843 | |||
SER.DMP. (TLC densitometric method) | ||||
|---|---|---|---|---|
Intact (µg/spot) | Acidic degradates (µg/spot) | Degradate % | Intact found (µg/spot) | Recovery % of Intacta |
5 | 20 | 80 | 4.99 | 99.96 |
10 | 15 | 60 | 10.07 | 100.07 |
12.5 | 12.5 | 50 | 12.51 | 100.21 |
15 | 10 | 40 | 14.98 | 99.95 |
20 | 5 | 20 | 19.60 | 99.85 |
Mean | 100.01 | |||
RSD% | 0.967 | |||