Table 3 Determination of DMP in laboratory prepared mixtures with its acidic degradation products by the developed HPLC and TLC procedures
DMP (HPLC method) | ||||
|---|---|---|---|---|
Intact (µg/ml) | Acidic degradates (µg/mL) | Degradate % | Intact found (µg/mL) | Recovery % of Intacta |
5 | 20 | 80 | 5.01 | 100.20 |
10 | 15 | 60 | 10.05 | 100.5 |
12.5 | 12.5 | 50 | 12.51 | 100.08 |
15 | 10 | 40 | 14.97 | 99.80 |
20 | 5 | 20 | 19.95 | 99.75 |
Mean | 100.06 | |||
RSD% | 0.918 | |||
DMP (TLC densitometric method) | ||||
|---|---|---|---|---|
Intact (µg/spot) | Acidic degradates (µg/spot) | Degradate % | Intact found (µg/spot) | Recovery % of Intacta |
5 | 20 | 80 | 4.95 | 99.00 |
10 | 15 | 60 | 10.01 | 100.10 |
12.5 | 12.5 | 50 | 12.53 | 100.24 |
15 | 10 | 40 | 14.99 | 99.93 |
20 | 5 | 20 | 19.90 | 99.5 |
Mean | 99.75 | |||
RSD% | 0.989 | |||