Table 4 Determination of SER.DMP in laboratory prepared mixtures with its basic degradation products by the developed HPLC and TLC procedures
HPLC method | ||||
|---|---|---|---|---|
Intact (µg/mL) | Basic degradates (µg/mL) | Degradate % | Intact found (µg/mL) | Recovery % of Intacta |
7.5 | 17.5 | 70 | 7.49 | 99.86 |
10 | 15 | 60 | 10.05 | 100.50 |
12.5 | 12.5 | 50 | 12.45 | 99.60 |
17.5 | 7.5 | 30 | 17.48 | 99.88 |
20 | 5 | 20 | 19.98 | 99.90 |
Mean | 99.94 | |||
RSD% | 0.911 | |||
TLC densitometric method | ||||
|---|---|---|---|---|
Intact (µg/spot) | Basic degradates (µg/spot) | Degradate % | Intact found (µg/spot) | Recovery % of Intacta |
7.5 | 17.5 | 70 | 7.48 | 99.73 |
10 | 15 | 60 | 9.95 | 99.50 |
12.5 | 12.5 | 50 | 12.52 | 100.16 |
17.5 | 7.5 | 30 | 17.49 | 99.94 |
20 | 5 | 20 | 19.95 | 99.75 |
Mean | 99.82 | |||
RSD% | 0.985 | |||