Table 5 Comparison of HPLC procedures for the Cfz determination
Mobile phase Reagent | Organic, (ratio %) | Column | Flow rate (mL/min) | Detection (nm) | pH adjustment | Retention time (min) | Refs. |
|---|---|---|---|---|---|---|---|
Potassium dihydrogen phosphate | ACN, 20 | ODS—150 mm × 4.6 mm, 5.0 µm | 1.0 | 230 | Without | 5.0 ± 0.1 | Current |
Tetraethylammonium acetate | MeOH, 35 | Bonded β-CD (Cyclobond I), 250 mm × 4.6 mm, 5.0 μm + postcolumn | 0.8 | 280 | 4.5 | 28.3 | [2] |
Tetramethylammonium hydroxide | MeOH, 25 | ODS—120 mm × 4.0 mm, 2.5 µm | 1.0 | 230 | 3.4 | 7.9 | [3] |
Phosphate buffer | MeOH, 55 | C18—250 mm × 4.6 mm, 5.0 µm  + Guard column | 0.85 | 260 | 3.2 | 23.7 | [22] |
Phosphate buffer | ACN, 65 | C8—150 mm × 4.6 mm, 5.0 μm | 1.0 | 215 | 3.5 | 4.2 | [23] |
Tetrabutyl ammonium hydroxide | ACN, 25 | C18—150 mm × 4.6 mm, 3.5 µm | 1.0 | 230 | 6.8 | 20 | [24] |
Ammonium acetate | ACN, 5 | C8—250 mm × 4.6 mm, 5.0 μm | 0.8 | 250 | 5.6 | 23.58 | [25] |