Skip to main content

Table 1 Chromatographic conditions and Parameters of system suitability of the proposed HPLC method for the determination of PAR and DAN

From: Hydro-organic mobile phase and factorial design application to attain green HPLC method for simultaneous assay of paracetamol and dantrolene sodium in combined capsules

Chromatographic conditions

Column

Hypersil C18 column

Mobile phase

40:60 (v/v) of ethanol: water, 0.2% TEA at pH 4.5 \(\pm\) 0.02 and 0.8 mL/min flowrate

Detection

UV detection 290 nm

Parameters of system suitability

Parameter

PAR

DAN

No of theoretical plates, N

1936

4500

Selectivity factor, α

12.66

Resolution, Rs

8.75

Retention time (tR), min

2.2

5.7

Tailing factor (T), 5% of the peak height

0.97

1.14

Asymmetry factor, 10% of the peak height

1.11

1.21

Run time, min

6

Back to article page

BMC Chemistry

ISSN: 2661-801X

Contact us