Hydro-organic mobile phase and factorial design application to attain green HPLC method for simultaneous assay of paracetamol and dantrolene sodium in combined capsules

Table 10 Eco-scale penalty points for the reported and the proposed HPLC methods [40]

Reported HPLC method [19]		Reported HPLC methods [20]		Suggested HPLC method
Reagents	Penalty points	Reagents	Penalty points	Reagents	Penalty points
Methanol	18	Phosphate buffer	0	Ethanol	0
Formic acid	6	Potassium dihydrogen phosphate	3	Water	0
Water	0	Methanol	18
	∑ 24		∑ 21		∑ 0

Instruments	Penalty points	Instruments	Penalty points	Instruments	Penalty points
HPLC–UV	1	HPLC–UV	1	HPLC–UV	1
Occupational hazard	0	Occupational hazard	0	Occupational hazard	0
Waste	12	Waste	9	Waste	4
	∑ 13		∑ 10		∑ 5
Total penalty points	37	Total penalty points	31	Total penalty points	5
Score	63	Score	69	Score	95

ISSN: 2661-801X