Table 4 Penalty points allotment and analytical eco-scale score calculation to assess the greenness of the present method
Reagents/Instrumentation | Amount | Amount’s penalty point | Hazard pictogram | Penalty points |
|---|---|---|---|---|
Reagents | ||||
KNO3 | 162.10 mg < 10 g | 1 | 1 | 2 |
HCl | 10–100 ml | 2 | 2 | 4 |
Water | > 100 ml | 3 | 0 | 0 |
Methanol | 10–100 ml | 2 | 3 | 6 |
Penta sulfonic acid sodium salt | 1.74g < 10g | 1 | 1 | 1 |
Instrumentation | ||||
HPLC-PDA (Hermetically sealed device & proper lab practice adopted by analyst) | 0 | |||
Sample preparation (storage and boiling water bath) | 3 | |||
Occupational hazard | 0 | |||
Waste | ||||
Unused sample extract & non-edible part of the radish | > 10g |  | 5 | |
No treatment | 3 | |||
Total Penalty points | 24 | |||
Analytical eco-scale score | 76 | |||