Fig. 2

Effect of different analytical columns on the separation of ribavirin in spiked chicken samples. A: Waters BEH amide column (2.1 mm×50 mm, 1.7 μm), B: Agilent ZORBAX SB–Aq column (4.6 mm×100 mm, 1.8 μm), C: Thermo Hypercarb column (2.1 mm×100 mm, 5 μm). Blank chicken matrices (M) were spiked with ribavirin at the level of 5 μg/kg (ES) with 50.0 ng/mL of internal standard (ISTD) ¹³C₅–ribavirin (IS). // indicated break of the time axis