LC–MS/MS separation and quantitation of ribavirin in chicken and comparison of different mass spectrometric platforms

Table 4 Stability of ribavirin under various conditions

Condition	Found (ng/mL)	RE (%)	CV (%)
Condition	QC low QC high	QC low QC high	QC low QC high
Freeze/thaw 120 h RT One − week storage	1.03 50.09 1.05 50.12 1.06 50.07	2.67 0.19 5.00 0.24 6.33 0.13	2.34 0.08 3.43 0.16 4.24 0.10

Freeze/thaw, 3 freeze (− 20 °C)/thaw cycles; one − week storage (20 °C). The initial concentrations of QC low/high samples were 1.00 ng/mL and 50.0 ng/mL, respectively. The concentration of internal standard (ISTD) ¹³C₅–ribavirin was 50.0 ng/mL. RE, relative error; CV, coefficient of variation; RT, room temperature (20 °C)

ISSN: 2661-801X