Table 2 System Suitability parameters of the proposed HPLC–DAD method

Selectivity (α)

1.37

1.70

1.11

1.07

1.24

1.11

1.25

α > 1

Resolution (R_s)

2.86

7.43

2.80

2.50

9.00

4.00

7.43

Rs > 1.5

Tailing factor (T)

1.15

1.23

0.95

1.06

0.97

1.07

0.98

1.14

T ≤ 2, T = 1 for symmetric peak

Column efficiency (N)

4806

3844

13766

36100

40000

55695

29122

23716

N > 2000

Height equivalent to theoretical plate (HETP) (cm/plate)

0.0052

0.0065

0.0018

0.0007

0.0006

0.0004

0.0009

0.0010

The smaller the value, the higher column efficiency

Retention time (T_R) (min ± 0.2)

5.21

6.16

8.82

9.51

10.04

11.89

12.84

15.02