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Table 4 Determination of the studied drugs in pharmaceutical dosage forms by the proposed HPLC–DAD method and application of standard addition technique

From: Sustainable chromatographic quantitation of multi-antihypertensive medications: application on diverse combinations containing hydrochlorothiazide along with LC–MS/MS profiling of potential impurities: greenness and whiteness evaluation

Pharmaceutical dosage form

HCT

VAL

AML

ATN

AMI

CAN

Exforge HCT® Tablets, B.N. A518682, Each tablet is labelled to contain 25.0 mg HCT, 160.0 mg VAL & 10.0 mg AML

% Found ± SD a

99.73 ± 0.88

99.47 ± 0.59

99.85 ± 0.56

Standard addition b % recovery of the pure added ± SD c

98.45 ± 0.77

99.98 ± 0.37

99.31 ± 0.30

Atenoretic® Tablets, B.N.71047, Each tablet is labelled to contain 25.0 mg HCT, 50.0mg ATN & 2.5 mg AMI

% Found ± SD a

99.66 ± 0.77

 

100.95 ± 0.25

99.13 ± 0.65

Standard addition b % recovery of the pure added ± SD d

100.54 ± 0.52

99.83 ± 1.03

99.41 ± 0.69

Atacand plus® Tablets, B.N. 19015, Each tablet is labelled to contain 12.5 mg HCT, & 16.0 mg CAN

% Found ± SD a

99.23 ± 0.57

98.80 ± 0.94

Standard addition b % recovery of the pure added ± SD e

98.73 ± 1.04

98.70 ± 0.68

  1. a Average of three experiments
  2. b Average of three experiments
  3. c Claimed Concentration; 32 µg/mL VAL, 1 µg/mL AML and 2.5 µg/mL HCT and pure added equivalent to 8.0, 16.0, 32.0 µg/mL VAL, 0.5, 1.0, 2.0 µg/mL AML and 1.0, 2.5, 5.0 µg/mL HCT
  4. d Claimed Concentration; 1.25 µg/mL AML, 25 µg/mL ATN and 12.5 µg/mL HCT and pure added equivalent to 0.5, 1.25,2.5 µg/mL AML,12.5, 25.0, 50.0 µg/mL ATN and 6.0, 12.5, 25.0 µg/mL HCT
  5. e Claimed Concentration; 16 µg/mL CAN and 12.5 µg/mL HCT and pure added equivalent to8.0, 16.0, 32.0 µg/mL CAN and 6.0, 12.5, 25.0 µg/mL HCT