Sustainable eco-friendly ratio-based spectrophotometric and HPTLC-densitometric methods for simultaneous analysis of co-formulated anti-migraine drugs with overlapped spectra

Table 1 The regression parameters and validation results for determination of ASP and MET by the developed methods

Parameters	HPTLC-densitometry		Spectrophotometric methods
	HPTLC-densitometry		RD		DRZC
	ASP	MET	ASP	MET	ASP	MET
Linearity (µg/band or µg/mL)	10–200	1–45	15–200	1.0–40	15–200	1.0–40
Slope	71.490	2871.3	0.006894	0.7983	0.006750	0.8559
Intercept	15129	4731.1	0.02435	− 0.3013	0.04388	− 0.9234
Correlation Coefficient(r)	0.9996	0.9996	0.9997	0.9994	0.9993	0.9993
Accuracy ^ab(Recovery% ± SD)	98.53 ± 1.47	98.36 ± 1.25	100.12 ± 1.36	99.54 ± 0.95	99.67 ± 1.68	100.20 ± 0.58
LOD (µg/band or µg/mL)	3.607	0.057	4.25	0.26	5.41	0.18
LOQ (µg/band or µg/mL)	9.02	0.76	14.9	0.93	14.4	0.73
Intra-day precision ^acRSD%	1.75	1.26	0.39	0.46	0.76	1.23
Inter-day precision ^acRSD%	1.96	1.63	0.50	1.05	0.98	1.70

^aAverage of three experiments
^b(n = 5 concentrations; for ASP (15,30,90,130,170 µg/band or µg/mL) and for MET (3,9,15,25,35 µg/band or µg/mL)
^c(n = 3 concentrations; for ASP (50,100,150 µg/band or µg/mL) and for MET (5, 15, 30 µg/band or µg/mL)

ISSN: 2661-801X