Table 6 Assessment of the green character for the developed methods using Eco-scale, GAPI and AGREE tools
Parameters | HPTLC-densitometry | Spectrophotometric methods | ||
|---|---|---|---|---|
Preparation | Reagent used: | Penalty points (PPs) | Solvents used: | Penalty points (PPs) |
| Â | Methanol | 12 | Methanol | 12 |
| Â | Cyclo-hexane | 8 | Â | |
| Â | methylene chloride | 1 | ||
Amount of reagents |  > 100 mL | 3 |  > 100 mL | 3 |
Instrument | HPTLC scanner and autosampler (enery used < 0.1 kWh per sample) | 0 | UV–Vis Spectrometry (energy used < 0.1 kWh per sample) | 0 |
Occupational hazards | Analytical process hermetization | 0 | Analytical process hermetization | 0 |
Waste | The waste volume is 1–10 mL per sample | 3 | The waste volume is 1–10 mL per sample | 3 |
Total penalty points | ∑ 27 | ∑ 18 | ||
Analytical Eco-Scale total score | 73 Acceptable green | 82 Excellent green | ||
GAPI |
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AGREE |
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