Table 1. Factors and responses for Box-Behnken experimental design of the optimized method
Std. | Run | Factor 1 | Factor 2 | Factor 3 | Response 1 | Response 2 |
|---|---|---|---|---|---|---|
A: Temperature | B: % ACN | C: pH | Rs | Rt | ||
15 | 1 | 30 | 65 | 5 | 3.22 | 2.514 |
14 | 2 | 30 | 65 | 5 | 3.22 | 2.514 |
13 | 3 | 30 | 65 | 5 | 3.22 | 2.514 |
10 | 4 | 30 | 70 | 3 | 2.25 | 1.785 |
8 | 5 | 35 | 65 | 7 | 1.11 | 2.714 |
1 | 6 | 25 | 60 | 5 | 3.84 | 3.416 |
3 | 7 | 25 | 70 | 5 | 2.12 | 1.81 |
9 | 8 | 30 | 60 | 3 | 4.25 | 3.318 |
2 | 9 | 35 | 60 | 5 | 1.05 | 3.205 |
5 | 10 | 25 | 65 | 3 | 4.54 | 2.874 |
12 | 11 | 30 | 70 | 7 | 1.82 | 1.824 |
4 | 12 | 35 | 70 | 5 | 1.98 | 1.802 |
11 | 13 | 30 | 60 | 7 | 0.98 | 4.281 |
16 | 14 | 30 | 65 | 5 | 3.22 | 2.514 |
6 | 15 | 35 | 65 | 3 | 3.08 | 2.415 |
17 | 16 | 30 | 65 | 5 | 3.22 | 2.514 |
7 | 17 | 25 | 65 | 7 | 0.88 | 3.198 |