Table 8 Ruggedness, Robustness, and stability of analytical solution of the proposed UPLC and UV methods
Parameter | UPLC | UV | Limit % | ||||
|---|---|---|---|---|---|---|---|
RS | MCR | ||||||
CFZ | PYD | CFZ | PYD | CFZ | PYD | ||
Day to Day | 0.82 | 0.93 | 0.63 | 0.81 | 0.56 | 0.77 | RSD ≤ 2.0% |
Analyst to Analyst | 0.78 | 1.20 | 0.55 | 0.79 | 0.71 | 0.89 | |
Column to Column | 0.54 | 1.12 | – | – | – | – | |
Flow rate change (± 0.1 mL/min) | 0.95 | 1.27 | – | – | – | – | |
pH changes of mobile phase (± 0.2) | 0.83 | 0.88 | – | – | – | – | |
Wavelength change (± 2.0) nm | 0.90 | 0.96 | 0.78 | 0.88 | 0.83 | 0.91 | |
Column temperature change (± 2.0) °C | 0.69 | 0.84 | – | – | – | – | |
Fresh Sample | 0.11 | 0.20 | 0.10 | 0.14 | 0.13 | 0.15 | |
Stored Sample in fridge | 0.43 | 0.56 | 0.23 | 0.31 | 0.25 | 0.34 | |
Stored Sample in room temperature | 0.74 | 0.89 | 0.45 | 0.52 | 0.47 | 0.66 | |