Table 1 Statistical analytical parameters for determination of ROS, ATO, EZE and SIM mixture using the proposed MEKC-DAD method

Wavelength (nm)

243

243

237

237

Concentration range (μg/mL)

10–100

10–100

10–100

10–100

Intercept (a)

− 0.493

− 0.393

− 7.167

4.104

S_a^a

0.283

0.520

1.224

0.668

Slope (b)

0.506

1.210

1.565

0.768

S_b^b

0.005

0.008

0.020

0.009

RSD% of the slope (S_b%)

0.99

0.66

1.28

1.17

Correlation coefficient (r)

0.9996

0.9998

0.9993

0.9995

S_y/x^c

0.414

0.762

1.792

1.037

F^d

12,336

20,842

6290

7662

Significance F

4.83 × 10^‒14

5.93 × 10^‒15

7.12 × 10^‒13

3.24 × 10^‒13

LOD^e (μg/mL)

0.52

0.75

0.42

0.64

LOQ^f (μg/mL)

1.73

2.50

1.40

2.13