Table 3 Robustness evaluation for the analysis of ROS, ATO, SIM and EZE using the proposed MEKC-DAD method

Buffer concentration (25 mM ± 2 mM)

20.93 ± 0.40

1.91

0.50

Buffer pH (9.2 ± 0.2)

21.37 ± 0.40

1.87

0.49

SDS concentration (25 mM ± 2 mM)

21.10 ± 0.36

1.71

0.25

%v/v acetonitrile (10 ± 2%)

21.43 ± 0.42

1.96

0.25

Wavelength (243 ± 2 nm)

21.20 ± 0.20

0.94

Buffer concentration (25 mM ± 2 mM)

60.90 ± 0.46

0.76

5.25 ± 0.02

0.38

Buffer pH (9.2 ± 0.2)

60.93 ± 0.40

0.66

5.33 ± 0.08

1.50

SDS concentration (25 mM ± 2 mM)

60.87 ± 0.40

0.66

5.37 ± 0.03

0.56

%v/v acetonitrile (10 ± 2%)

61.07 ± 0.70

1.15

5.37 ± 0.04

0.75

Wavelength (243 ± 2 nm)

61.00 ± 0.30

0.49

Buffer concentration (25 mM ± 2 mM)

60.90 ± 0.36

0.59

8.01 ± 0.09

1.12

Buffer pH (9.2 ± 0.2)

61.17 ± 0.35

0.57

8.09 ± 0.07

0.87

SDS concentration (25 mM ± 2 mM)

61.10 ± 0.20

0.33

8.08 ± 0.03

0.37

%v/v acetonitrile (10 ± 2%)

61.03 ± 0.35

0.57

8.00 ± 0.04

0.50

Wavelength (237 ± 2 nm)

61.20 ± 0.20

0.33

Buffer concentration (25 mM ± 2 mM)

63.12 ± 0.25

0.40

8.64 ± 0.07

0.81

Buffer pH (9.2 ± 0.2)

62.92 ± 0.19

0.30

8.63 ± 0.10

1.16

SDS concentration (25 mM ± 2 mM)

63.10 ± 0.36

0.57

8.61 ± 0.06

0.70

%v/v acetonitrile (10 ± 2%)

62.90 ± 0.46

0.73

8.65 ± 0.10

1.16

Wavelength (237 ± 2 nm)

63.18 ± 0.23

0.36