Green wastewater treatment of repurposed COVID-19 therapy (levofloxacin) using synthesized magnetite pectin nanoparticles, comparison with mesoporous silica nanoparticles

Table 3 System suitability and validation parameters for the proposed HPLC/UV method

Parameters		HPLC	Reference values^*
Linearity (µg/mL)		10–100
Correlation coefficient (r)		0.9997
Slope		23.784
Intercept		22.649
Standard deviation of residuals from line		21.325
LOD (µg/mL)		2.959
LOQ (µg/mL)		8.966
Accuracy (Recovery % ± SD)^a		101.03 ± 0.86
Precision (RSD)	Intraday^b	99.36 ± 0.97
Precision (RSD)	Interday^c	100.11 ± 1.33
t_R, min		3.916 ± 0.05
Tailing factor (T)		0.78	T ≤ 2, T = 1 for symmetric peak
Capacity factor (Kʹ)		1.42	Kʹ = 1–10 acceptable
Plates number (N)		6255	N > 2000
Height equivalent to theoretical plate (HETP; cm plate⁻¹)		0.03	The smaller the value, the higher the column efficiency

^aAverage of three blind concentrations analyzed in triplicate
^bAverage of three concentrations (25, 50, 75 µg/mL) analyzed in triplicate on the same day
^cAverage of three concentrations (25, 50, 75 µg/mL) analyzed in triplicate on three successive days
* according to USP

ISSN: 2661-801X