Table 1 Gradient programme of the HPLC method for determination of MUP, FLU, Pseud-D, and FIC
Time (min) | Buffer (%) | Methanol (%) | Flow rate(mL/min) |
|---|---|---|---|
0–7 min | 50% | 50% | 1mL/min |
8–15 min | 20% | 80% | 1mL/min |
16–22 min | 50% | 50% | 1mL/min |
Time (min) | Buffer (%) | Methanol (%) | Flow rate(mL/min) |
|---|---|---|---|
0–7 min | 50% | 50% | 1mL/min |
8–15 min | 20% | 80% | 1mL/min |
16–22 min | 50% | 50% | 1mL/min |