Greenness assessment of two chromatographic methods developed for the determination of Mupirocin in two binary mixtures along with its impurity

Table 2 Parameters required for system suitability tests of the proposed HPTLC method

Parameters	Mixture (1)				Mixture (2)			Reference values [24]
Parameters	Pseud-D	MUP	FIC	FLU	Pseud-D	MUP	MF	Reference values [24]
Retardation factor (R_f )	0.15	0.42	0.55	0.74	0.14	0.39	0.68
Resolution (Rs)	2.18	1.52	3.8		1.8	2.32		Rs > 1.5
Tailing factor (T)	0.83	0.95	0.83	0.84	0.8	1	0.83
Retention factor (k’) ^a	5.66	1.38	0.818	0.35	6.14	1.56	0.47	1–5
Selectivity factor (α)	4.10	1.68	2.33		3.93	3.32		α > 1
Column efficiency (N) ^b	1024	711	4096	3505	1600	256	4096
Height equivalent to theoretical plate (HETP) (mm)	0.0007	0.001	0.0001	0.0002	0.0005	0.0031	0.0002	The smaller the value, the higher the column efficiency

^aα = k2/k1, where k is the capacity factor: k = (1 − R_f)/R_f.^bRs = [2 (Rf2 − Rf1)]/ (W1 + W2), where Rf is retardation factor and W is peak width at 5% from the baseline of the peak height
^CN = 16 (z/w) ², where z is the migration length of the spot and w is the spot width

ISSN: 2661-801X