Table 3 Parameters required for system suitability tests of the proposed HPLC method
Parameters | Mixture (1) | Mixture (2) | Reference value [25] | |||||
|---|---|---|---|---|---|---|---|---|
FIC | Pseud-D | MUP | FLU | Pseud-D | MUP | MF | ||
Retention time (tR) | 2.78 | 5.37 | 12.36 | 13.70 | 2.37 | 3.40 | 4.73 | |
Resolution (Rs) | 7.77 | 22.1 | 7.16 | 5.34 | 5.81 | ≥ 1.5 | ||
Tailing factor (T) | 1.11 | 1.18 | 1.23 | 1.20 | 1.18 | 1.17 | 1.18 | T = 1 for typical symmetric peak |
Retention factor (k) | 1.05 | 2.98 | 8.15 | 9.14 | 2.85 | 1.52 | 2.5 | 1–5 |
Selectivity factor (α) | 2.81 | 2.73 | 1.12 | 2.01 | 1.64 | > 1 | ||
Column efficiency (N) | 3270 | 2110 | 101,000 | 75,100 | 2604 | 4779 | 67,600 | Increases with efficiency of the separation |
Height equivalent to theoretical plates (HETP) | 0.0076 | 0.0118 | 0.00024 | 0.00033 | 0.0096 | 0.0052 | 0.0003 | The smaller the value, the higher the column efficiency |