Table 4 Parameters and validation sheet for determination of the cited compounds by the proposed HPTLC method

Linearity range

2–40 µg/band

0.1-2 µg/band

0.1-4 µg/band

0.1-2 µg/ band

0.2-4 µg/ band

Accuracy

Mean ± RSD

101.29± 0.677

99.59 ± 1.146

98.76 ± 1.670

100.61 ± 0.519

99.80 ± 1.447

Correlation coefficient (r)

0.9992

0.9997

0.9999

0.9999

0.9998

Slope

1399.3

3449.4

2997.8

6338.4

872.69

Standard error [SE] of slope

114.31

148.45

211.89

25.36

6.57

Intercept

4403.9

765.75

1961.5

777.19

26

Standard error [SE] of intercept

2504.61

179.99

384.08

23.63

12.53

Precision

Repeatability ^a

(%RSD)

1.788

0.718

1.475

1.018

0.516

Intermediate precision ^b

(%RSD)

1.616

1.00

0.968

1.119

1.159

Robustness*

(RSD %)

0.183

1.23

0.633

0.383

1.508

LOD

0.358 µg/band

0.006 µg/ band

0.012 µg/ band

0.030 µg/ band

0.029 µg/ band

LOQ

1.086 µg/ band

0.020 µg/ band

0.037 µg/ band

0.091 µg/ band

0.089 µg/ band