Greenness assessment of two chromatographic methods developed for the determination of Mupirocin in two binary mixtures along with its impurity

Table 5 Parameters and validation sheet for determination of the cited compounds by the proposed HPLC method

Parameter	Mixture (1)				Mixture (2)
Parameter	MUP	FLU	Pseud-D	FIC	MUP	MF	Pseud-D
Linearity range	80–800 µg/mL	0.5–50 µg/mL	1–16 µg/mL	0.4–12 µg/mL	80–800 µg/mL	1–20 µg/mL	1–16 µg/mL
Accuracy Mean ± RSD	98.72± 1.772	100.11 ± 0.688	98.93 ± 1.335	99.72 ± 0.634	99.08± 1.75	100.78 ± 0.708	99.60 ± 0.769
Correlation coefficient (r)	0.9995	0.9999	0.9991	0.9997	0.9997	0.9997	0.9999
Slope	13,525	25,749	60,486	28,757	13,530	25,388	60,509
Standard error [SE] of slope	137.27	110.29	890.66	265.98	110.13	209.06	351.25
Intercept	279,718	5790	13,149	408.31	237,931	471.67	21,505
Standard error [SE] of intercept	58961.25	2648.21	8267.73	1881.27	47305.33	2350.78	3260.52
Precision Repeatability ^a (%RSD)	1.38	1.00	2.00	1.22	1.816	1.411	1.167
Intermediate precision ^b (%RSD)	0.679	0.947	1.51	1.28	1.90	1.382	1.212
LOD	7.208 µg/mL	0.087 µg/mL	0.322 µg/ mL	0.014 µg/ mL	3.449 µg/mL	0.016 µg/mL	0.068 µg/ mL
LOQ	21.842 µg/mL	0.265 µg/ mL	0.976 µg/ mL	0.044 µg/ mL	10.452 µg/mL	0.05 µg/ mL	0.210 µg/ mL

^a average of three different concentrations repeated three times within the day
^b average of three different concentrations repeated three times in three successive days

ISSN: 2661-801X