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Table 3 Assay parameters and validation data for determination of ibuprofen and phenylephrine by the proposed HPLC method

From: Development of an eco-friendly HPLC method for the stability indicating assay of binary mixture of ibuprofen and phenylephrine

 

PHE

IBU

Range (µg/mL)

0.3–10

10–100

Slope

989,994

1611.40

Intercept

528,735

− 11,978

SE of the slope

14,376.11

18.4132

SE of the intercept

68,432.38

1143.568

Correlation coefficient (r)

0.9995

0.9997

LODa (µg/mL)

0.4399

2.75

LOQa (µg/mL)

0.30

8.33

Accuracyb

99.44%

99.45%

Repeatabilityc

1.34

1.09

Intermediate precisiond

1.47

1.23

Specificity (mean ± SD)e

99.67 ± 1.67

100.75 ± 1.44

Robustness

1.94

1.19

Flow rate 1.0 + 0.1 mL/min

  

Mobile phase; 80:20, 60:40

  

80.8:20.2, 60.6:40.4

1.87

1.31

  1. aLimits of detection and quantitation are determined via calculations, LOD = 3.3 σ/S (SD of the residuals/slope), LOQ = 10 σ/S (SD of the residuals/slope), where σ is the standard deviation of y-intercepts of regression lines and S is the slope of the calibration curves
  2. bMean (n = 3) R% of three concentrations within the linearity ranges
  3. cIntra-day precision (n = 3), three concentrations repeated three times within the same day
  4. dInter-day precision (n = 3), three concentrations repeated three times in three successive days
  5. eMean (n = 3) R% of laboratory prepared mixtures containing IBU, PHE and its degradation products
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BMC Chemistry

ISSN: 2661-801X

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