Parameter | RP-HPLC | TLC | Reference values | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
SAC | MTP | DMA | LDC | MIC | SAC | LDC | MIC | MTP | HPLC | TLC | |||
Retention time (tR) / Retardation factor | 1.60 | 2.19 | 2.52 | 3.25 | 7.38 | 0.12 | 0.30 | 0.42 | 0.54 | – |  | ||
Tailing factor (T) | 1.05 | 1.00 | 0.88 | 0 .95 | 1.09 | 1.18 | 1.00 | 0.79 | 1.00 | T = 1 for a symmetric peak | T = 1 for a symmetric peak | ||
Capacity factor (K`) | 1.30 | 2.12 | 2.60 | 3.63 | 9.55 | 7.33 | 2.33 | 1.38 | 0.85 | 1–10 | The higher the capacity factor, the smaller the Rf | ||
Resolution factor (Rs) | 10.37 | 3.85 | 2.21 | 3.20 |  | 1.23 | 1.00 | 1.02 |  | Rs > 2 | Rs > 1 | ||
Selectivity factor (α) | 2.63 | 1.63 | 1.23 | 1.40 |  | 3.15 | 1.69 | 1.62 |  | α > 1 | α > 1 | ||
Number of theoretical plates (N) | 1840 | 3401 | 4508 | 1863 | 3492 | Â | Â | Â | Â | Increase with the efficiency of the separation | Â | ||
HETP | 0.136 | 0.074 | 0.055 | 0.134 | 0.072 | Â | Â | Â | Â | The smaller the value, the higher the column efficiency | Â |