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Table 1 System suitability parameters of the proposed RP-HPLC and TLC methods

From: Green chromatographic methods for determination of co-formulated lidocaine hydrochloride and miconazole nitrate along with an endocrine disruptor preservative and potential impurity

Parameter

RP-HPLC

TLC

Reference values

SAC

MTP

DMA

LDC

MIC

SAC

LDC

MIC

MTP

HPLC

TLC

Retention time (tR) / Retardation factor

1.60

2.19

2.52

3.25

7.38

0.12

0.30

0.42

0.54

–

 

Tailing factor (T)

1.05

1.00

0.88

0 .95

1.09

1.18

1.00

0.79

1.00

T = 1 for a symmetric peak

T = 1 for a symmetric peak

Capacity factor (K`)

1.30

2.12

2.60

3.63

9.55

7.33

2.33

1.38

0.85

1–10

The higher the capacity factor, the smaller the Rf

Resolution factor (Rs)

10.37

3.85

2.21

3.20

 

1.23

1.00

1.02

 

Rs > 2

Rs > 1

Selectivity factor (α)

2.63

1.63

1.23

1.40

 

3.15

1.69

1.62

 

α > 1

α > 1

Number of theoretical plates (N)

1840

3401

4508

1863

3492

    

Increase with the efficiency of the separation

 

HETP

0.136

0.074

0.055

0.134

0.072

    

The smaller the value, the higher the column efficiency

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