Table 2 Assay validation parameters of the proposed RP-HPLC and TLC methods

Linearity

 Range

2.00–100.00 µg/mL

1.00–100.00 µg/mL

1.00–20.00 µg/mL

1.00–20.00 µg/mL

0.30–3.00 µg/band

0.30–3.00 µg/band

 Intercept

46.441

4.4143

−1.4165

65.621

349.67

1217.5

 Slope

64.967

66.568

89

125.33

1120.5

4718

 Correlation coefficient (r)

0.9999

0.9999

0.9999

1

1

1

Precision(± %RSD)

 Repeatabiliy^a

0.881

0.869

0.50

1.133

0.862

1.228

 Intermediate precision^b

1.193

1.067

0.531

1.23

1.866

1.72

 Accuracy^c

Mean

% RSD

101.04

0.782

101.31

0.936

99.62

1.371

99.91

1.302

101.1

1.658

100.72

0.667

 Specificity^d

Mean

% RSD

100.16

1.078

100.37

1.71

99.91

0.856

99.73

0.84

99.63

0.964

100.1

0.547

 LOD^e

–

–

0.231

0.084

–

–

 LOQ^e

–

–

0.70

0.255

–

–

 Robustness parameter^f (%RSD)

1.054

1.247

1.28

1.423

0.765

1.427