Green chromatographic methods for determination of co-formulated lidocaine hydrochloride and miconazole nitrate along with an endocrine disruptor preservative and potential impurity

Table 4 Statistical comparison between the proposed methods and a reported HPLC method for the determination of LDC and MIC

Value	Proposed TLC method		Reported method ^a (Dosage form)		Proposed RP-HPLC method		Reported method (Pure form)
Value	LDC	MIC	LDC	MIC	LDC	MIC	LDC	MIC
Mean	99.49	98.8	98.89	99.24	101.04	100.94	100.53	98.9
SD	0.878	0.4	1.438	0.86	0.79	1.741	1.66	1.048
N	3	3	5	5	5	5	5	5
Variance	0.877	0.4	2.0674	0.7397	0.624	3.031	2.756	1.098
Student’s t-test ^b (2.447)	0.636	0.761			0.620	2.245
F value^b (19.25)	2.36	1.85			4.42	2.76

^aGradient HPLC–DAD stability indicating determination of miconazole nitrate and lidocaine hydrochloride in their combined oral gel dosage form
^bFigures in parentheses are the corresponding tabulated values at p = 0.05

ISSN: 2661-801X