Table 5 Comparison of the greenness profiles of proposed methods and the reported one using NEMI, GAPI, and AGREE tools

Proposed RP-HPLC method

The method was developed by using Waters X Select® CSHTMC18 column and a gradient mobile phase composed of methanol and phosphate buffer (pH 6) in different ratios. The flow rate was 1.5 mL/min and detection limit was 210 nm

Proposed TLC method

The method was developed by using aluminum TLC plates precoated with silica gel 60F254 as the stationary phase, mobile phase was consisting of ethyl acetate: methanol: formic acid (9:1:0.1, v/v/v) and detection was performed at 220.0 nm

Reported metho

The method was developed using C8 column with gradient elution of the mobile phase composed of 0.05 M phosphoric acid and acetonitrile, at a flow rate of 1 mL/min and the multiple wavelength detector was set at 215 nm