Fig. 8

In vivo antitumor effect of compound 3h. (A-E) A549 cells were injected into the flanks of nude mice. When the tumor volume reached approximately 100 mm³, the nude mice were sorted into two groups (n = 5) and one group was intraperitoneally injected with 3h (2.5mg/kg, once a day) for 14 days. (A) Images of sacrificed mice and excised tumors in each group. (B) The development of tumor size during the treatment period was recorded. (C) At the end of the experiment, tumors were resected and weighed. (D) Western blotting analysis of the expression of Cyclin D1, c-Caspase3, Bax, and Bcl2 in total tumor lysates from vehicle- and 3h-treated mice (n = 3). β-actin was used as a loading control. (E) Expression levels were quantitated using ImageJ software. Full-length gels are presented in Supplementary Fig. S2. All data are expressed as mean ± SEM. ^***p < 0.001, ^**p < 0.01, ^*p < 0.05 compared to the control group