Fig. 9

MTT assay and TUNEL staining in MPC and AML-12 cells. (A) MPC cells were cultured with 20 µmol cisplatin or 20 µmol cisplatin combined with 0.01, 0.1, 1, and 10 µmol 3h for 12 h, and cell viability was determined by MTT test. (B) Representative images and quantification of TUNEL staining of MPC cells treated with 20 µmol cisplatin or 20 µmol cisplatin combined with 1 µmol 3h for 12 h. Scale bars = 100 μm. (C) AML-12 cells were cultured with 20 µmol cisplatin or 20 µmol cisplatin combined with 0.01, 0.1, 1, 10 µmol 3h for 12 h, and cell viability was determined by MTT assay. (D) Representative images and quantification of TUNEL staining of AML-12 cells treated with 20 µmol cisplatin or 20 µmol cisplatin and 1 µmol 3h for 24h. Scale bars = 100 μm. n = 4 per group. All data are expressed as mean ± SEM. ^***p < 0.001, ^**p < 0.01 compared to the Cis group