Table 5 Determination of ASP, ATV and CLP in laboratory prepared mixtures using the developed HPLC–DAD method
Taken (μg/mL) | AUP | Found (μg/mL) | Recovery % | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
ASP | ATV | CLP | ASP | ATV | CLP | ASP | ATV | CLP | ASP | ATV | CLP |
1.5 | 0.1 | 0.75 | 2.083 | 0.295 | 0.586 | 1.493 | 0.100 | 0.753 | 99.53 | 100.00 | 100.40 |
5 | 0.5 | 5 | 5.991 | 1.031 | 3.849 | 4.938 | 0.494 | 4.995 | 98.76 | 98.80 | 99.90 |
7.5 | 1 | 7.5 | 8.876 | 1.96 | 5.789 | 7.481 | 0.993 | 7.516 | 99.75 | 99.30 | 100.21 |
15 | 2 | 7.5 | 17.255 | 3.805 | 5.775 | 14.866 | 1.982 | 7.498 | 99.11 | 99.10 | 99.97 |
25 | 5 | 25 | 28.593 | 9.323 | 19.269 | 24.860 | 4.942 | 25.039 | 99.44 | 98.84 | 100.16 |
50 | 10 | 35 | 57.128 | 18.567 | 26.963 | 50.012 | 9.901 | 35.040 | 100.02 | 99.01 | 100.11 |
Mean | 99.44 | 99.18 | 100.13 | ||||||||
± SD | 0.451 | 0.443 | 0.178 | ||||||||
± RSD% | 0.454 | 0.447 | 0.178 | ||||||||