Table 6 Determination of ASP, ROS and CLP in laboratory prepared mixtures using the developed HPLC–DAD method
Taken (μg/mL) | AUP | Found (μg/mL) | Recovery % | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
ASP | ROS | CLP | ASP | ROS | CLP | ASP | ROS | CLP | ASP | ROS | CLP |
1.5 | 0.1 | 0.75 | 2.077 | 0.128 | 0.583 | 1.488 | 0.100 | 0.749 | 99.20 | 100.00 | 99.87 |
5 | 0.5 | 5 | 6.007 | 0.533 | 3.831 | 4.952 | 0.494 | 4.971 | 99.04 | 98.80 | 99.42 |
7.5 | 1 | 7.5 | 8.873 | 1.048 | 5.752 | 7.478 | 0.996 | 7.468 | 99.71 | 99.60 | 99.57 |
15 | 2 | 7.5 | 17.278 | 2.089 | 5.752 | 14.886 | 2.009 | 7.468 | 99.24 | 100.45 | 99.57 |
25 | 5 | 25 | 28.722 | 5.125 | 19.21 | 24.974 | 4.964 | 24.962 | 99.90 | 99.28 | 99.85 |
50 | 10 | 35 | 57.100 | 10.334 | 26.953 | 49.987 | 10.035 | 35.027 | 99.97 | 100.35 | 100.08 |
Mean | 99.51 | 99.75 | 99.73 | ||||||||
± SD | 0.398 | 0.642 | 0.244 | ||||||||
± RSD% | 0.400 | 0.643 | 0.245 | ||||||||