Table 8 ESA, NEMI, GAPI and AGREE tools for greenness assessment of recently published chromatographic methods and the developed method for simultaneous determination of ASP, CLP and ATV

The developed method

Reagents

Penalty Points

Acetonitrile

4

water

0

phosphate buffer

0

Phosphoric acid

2

Σ

6

HPLC/UPLC

1

Waste

3

Occupational hazards

0

Σ

4

Total penalty points

10

ESA score

90

[11]

Reagents

Acetonitrile

4

Methanol

6

Triethylamine

6

Phosphoric acid

2

Σ

18

HPLC/UPLC

1

Waste

3

Occupational hazards

0

Σ

4

Total penalty points

22

ESA score

78

[12]

Reagents

Acetonitrile

4

Phosphate buffer

0

Phosphoric acid

2

Σ

6

HPLC/UPLC

1

Waste

3

Occupational hazards

0

Σ

4

Total penalty points

10

ESA score

90

[13]

Reagents

Acetonitrile

4

Methanol

6

Phosphate buffer

0

Phosphoric acid

2

Σ

12

HPLC/UPLC

1

Waste

3

Occupational hazards

0

Σ

4

Total penalty points

16

ESA score

84

[14]

Reagents

Acetonitrile

4

Methanol

6

Water

0

Phosphoric acid

2

Σ

12

HPLC/UPLC

1

Waste

3

Occupational hazards

0

Σ

4

Total penalty points

16

ESA score

84

[15]

Reagents

Acetonitrile

4

Phosphate buffer

0

Phosphoric acid

2

Σ

6

HPLC/UPLC

1

Waste

3

Occupational hazards

0

Σ

4

Total penalty points

10

ESA score

90