Table 9 ESA, NEMI, GAPI and AGREE tools for greenness assessment of recently published chromatographic methods and the developed method for simultaneous determination of ASP, CLP and ROS

The developed method

Reagents

Penalty Points

Acetonitrile

4

water

0

phosphate buffer

0

Phosphoric acid

2

Σ

6

HPLC/UPLC

1

Waste

3

Occupational hazards

0

Σ

4

Total penalty points

10

ESA score

90

[21]

Reagents

Acetonitrile

4

water

0

phosphate buffer

0

acetic acid

2

Σ

6

HPLC/UPLC

1

Waste

3

Occupational hazards

0

Σ

4

Total penalty points

10

ESA score

90

[22]

Reagents

Methanol

6

water

0

Phosphoric acid

2

Σ

8

HPLC/UPLC

1

Waste

3

Occupational hazards

0

Σ

4

Total penalty points

12

ESA score

88

[23]

Reagents

Acetonitrile

4

water

0

phosphate buffer

0

Phosphoric acid

2

Σ

6

HPLC/UPLC

1

Waste

3

Occupational hazards

0

Σ

4

Total penalty points

10

ESA score

90