Ecofriendly single-step HPLC and TLC methods for concurrent analysis of ternary antifungal mixture in their pharmaceutical products

Table 1 Regression and validation parameters of the proposed HPLC and HPTLC methods for determination of MIC, NYS, and MET

Parameters	HPLC			HPTLC
Parameters	MIC	NYS	MET	MIC	NYS	MET
Linearity	5–50 μg/mL	4–50 μg/mL	4–40 μg/mL	0.4–2 μg/band	0.4–2.2 μg/band	0.4–2 μg/band
Slope	13.9680	4.3515	28.2340	0.4762	0.8460	1.102
Intercept	− 0.9486	193.2800	3.8488	0.2186	− 0.2224	0.4583
Correlation coefficient	0.9999	0.9999	0.9999	0.9999	0.9998	0.9999
Accuracy (%)	100.41	100.42	99.19	99.53	100.28	100.00
Repeatability (RSD%)^a	0.17	0.62	0.61	0.80	0.49	0.77
Intermediate Precision (RSD%)^b	0.71	0.82	0.66	1.33	0.67	1.69
LOD^c	1.19 μg/mL	0.95 μg/mL	0.80 μg/mL	0.11 μg/band	0.09 μg/band	0.07 μg/band
LOQ^c	3.62 μg/mL	2.87 μg/mL	2.43 μg/mL	0.34 μg/band	0.27 μg/band	0.22 μg/band

^aThe intraday precision (n = 3), average of three different concentrations repeated three times within day
^bThe interday precision (n = 3), average of three different concentrations repeated three times in three successive days
^cLimit of detection and quantitation are determined via calculations: LOD = (SD of the response/slope) × 3.3; LOQ = (SD of the response/slope) × 10

ISSN: 2661-801X