Ecofriendly single-step HPLC and TLC methods for concurrent analysis of ternary antifungal mixture in their pharmaceutical products

Table 4 Statistical comparison of the results obtained by the proposed methods and the reported method for determination of MIC, MET and NYS in their pharmaceutical formulation

Parameters	HPLC method		HPTLC method		Reported method [6]
Parameters	MIC	NYS	MIC	NYS	MIC	NYS
Monicure plus^® vaginal suppository
Mean %	102.28	101.28	102.03	101.03	102.37	101.01
SD	0.33	0.66	0.54	0.44	0.43	0.65
n	6	6	6	6	6	6
Student’s t-test (2.228)^a	0.410	0.710	1.210	0.080	–	–
F-value (5.050)^a	1.720	1.010	1.560	2.220	–	–

Parameters	HPLC method		HPTLC method		Reported method [10]
Parameters	MET	NYS	MET	NYS	MET	NYS
Amrizole N^® vaginal suppository
Mean %	102.01	99.08	102.32	99.11	102.39	99.08
SD	0.62	0.57	0.27	0.86	0.31	0.59
n	6	6	6	6	6	6
Student’s t-test (2.228)^a	1.380	0.002	0.430	0.070	–	–
F-value (5.050)^a	3.940	1.060	1.290	2.130	–	–

^aFigures in parentheses represent the corresponding tabulated of t and F at P = 0.05

ISSN: 2661-801X